Measurement of density and affinity for dopamine D 2 receptors by a single positron emission tomography scan with multiple injections of 11 Craclopride

Yoko Ikoma, Hiroshi Watabe, Takuya Hayashi, Yoshinori Miyake, Noboru Teramoto, Kotaro Minato, Hidehiro Iida

研究成果: Article査読

8 被引用数 (Scopus)

抄録

Positron emission tomography (PET) with 11 Craclopride has been used to investigate the density (Bmax) and affinity (Kd) of dopamine D2 receptors related to several neurological and psychiatric disorders. However, in assessing the Bmax and Kd, multiple PET scans are necessary under variable specific activities of administered 11 Craclopride, resulting in a long study period and unexpected physiological variations. In this paper, we have developed a method of multiple-injection graphical analysis (MI-GA) that provides the Bmax and Kd values from a single PET scan with three sequential injections of 11 Craclopride, and we validated the proposed method by performing numerous simulations and PET studies on monkeys. In the simulations, the three-injection protocol was designed according to prior knowledge of the receptor kinetics, and the errors of Bmax and K d estimated by MI-GA were analyzed. Simulations showed that our method could support the calculation of B max and Kd, despite a slight overestimation compared with the true magnitudes. In monkey studies, we could calculate the Bmax and Kd of diseased or normal striatum in a 150 mins scan with the three-injection protocol of 11 Craclopride. Estimated Bmax and K d values of D2 receptors in normal or partially dopamine-depleted striatum were comparable to the previously reported values.

本文言語English
ページ(範囲)663-673
ページ数11
ジャーナルJournal of Cerebral Blood Flow and Metabolism
30
3
DOI
出版ステータスPublished - 2010 3月
外部発表はい

ASJC Scopus subject areas

  • 神経学
  • 臨床神経学
  • 循環器および心血管医学

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