TY - JOUR
T1 - Map-based cloning of a candidate gene conferring fusarium yellows resistance in Brassica oleracea
AU - Shimizu, Motoki
AU - Pu, Zi Jing
AU - Kawanabe, Takahiro
AU - Kitashiba, Hiroyasu
AU - Matsumoto, Satoru
AU - Ebe, Yusuke
AU - Sano, Monari
AU - Funaki, Taketo
AU - Fukai, Eigo
AU - Fujimoto, Ryo
AU - Okazaki, Keiichi
N1 - Funding Information:
Acknowledgments The authors sincerely thank Dr. Y. Kadota at the National Agriculture and Food Research Organization/NARO Tohoku Agricultural Research Center, Japan, for kindly providing Cong: 1-1 strain of F. oxysporum f. sp. conglutinans. This work was supported in part by Research Fellowships of JSPS for Young Scientists for M. Shimizu, the Programme for Promotion of Basic and Applied Researches for Innovations in Bio-oriented Industry, and the Adaptable and Seamless Technology Transfer Program through target-driven R&D from Japan Science and Technology Agency (JST) to K. Okazaki.
Funding Information:
The authors sincerely thank Dr. Y. Kadota at the National Agriculture and Food Research Organization/NARO Tohoku Agricultural Research Center, Japan, for kindly providing Cong: 1-1 strain of F. oxysporum f. sp. conglutinans. This work was supported in part by Research Fellowships of JSPS for Young Scientists for M. Shimizu, the Programme for Promotion of Basic and Applied Researches for Innovations in Bio-oriented Industry, and the Adaptable and Seamless Technology Transfer Program through target-driven R&D from Japan Science and Technology Agency (JST) to K. Okazaki.
Publisher Copyright:
© Springer-Verlag Berlin Heidelberg 2014.
PY - 2015/1
Y1 - 2015/1
N2 - Yellow wilt disease is one of the most serious diseases of cabbage worldwide. Type A resistance to the disease is controlled by a single dominant gene that is used in cabbage breeding. Our previous QTL study identified the FocBo1 locus controlling type A resistance. In this study, the FocBo1 locus was fine-mapped by using 139 recombinant F2 plants derived from resistant cabbage (AnjuP01) and susceptible broccoli (GCP04) DH lines. As a result, we successfully delimited the location of FocBo1 within 1.00 cM between markers, BoInd 2 and BoInd 11. Analysis of BAC and cosmid sequences corresponding to the FocBo1 locus identified an orthologous gene of Bra012688 that was recently identified as an candidate gene that confers yellows resistance in Chinese cabbage. The candidate gene-specific DNA markers and phenotypes in F1 cabbage cultivars and their selfed F2 populations showed a perfect correlation. Our identification of the candidate gene for FocBo1 will assist introduction of fusarium resistance into B. oleracea cultivars and contribute further understanding of interaction between Brassica plants and fusarium.
AB - Yellow wilt disease is one of the most serious diseases of cabbage worldwide. Type A resistance to the disease is controlled by a single dominant gene that is used in cabbage breeding. Our previous QTL study identified the FocBo1 locus controlling type A resistance. In this study, the FocBo1 locus was fine-mapped by using 139 recombinant F2 plants derived from resistant cabbage (AnjuP01) and susceptible broccoli (GCP04) DH lines. As a result, we successfully delimited the location of FocBo1 within 1.00 cM between markers, BoInd 2 and BoInd 11. Analysis of BAC and cosmid sequences corresponding to the FocBo1 locus identified an orthologous gene of Bra012688 that was recently identified as an candidate gene that confers yellows resistance in Chinese cabbage. The candidate gene-specific DNA markers and phenotypes in F1 cabbage cultivars and their selfed F2 populations showed a perfect correlation. Our identification of the candidate gene for FocBo1 will assist introduction of fusarium resistance into B. oleracea cultivars and contribute further understanding of interaction between Brassica plants and fusarium.
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U2 - 10.1007/s00122-014-2416-6
DO - 10.1007/s00122-014-2416-6
M3 - Article
C2 - 25351523
AN - SCOPUS:84930008557
VL - 128
SP - 119
EP - 130
JO - Theoretical And Applied Genetics
JF - Theoretical And Applied Genetics
SN - 0040-5752
IS - 1
ER -