Macrophage with gold nanorod visualized by optical-resolution and acoustic-resolution photoacoustic microscopes

Rena Yamazaki, Koetsu Ogasawara, Mitsuhiro Fujiwara, Kazuto Kobayashi, Yoshifumi Saijo

研究成果: Conference contribution

2 被引用数 (Scopus)

抄録

Macrophages play a key role in inflammation and they are frequently observed in vulnerable atherosclerotic plaque. In the present study, macrophages phagocytosing gold nanorod (AuNR) were observed by optical-resolution (OR) and acoustic-resolution (AR) photoacoustic microscope (PAM). The OR-PAM consisted of diode laser optically focused to 60 micron and planar ultrasonic transducer with the central frequency of 8 MHz placed under the object. AR-PAM consisted of concave ultrasonic transducer with the central frequency of 20 MHz and optical fiber through the center hole of the transducer for laser irradiation. First, PA signal from gold, silver and copper wire were measured in order to determine the best metal substrate for enhancing PA contrast. Gold generated largest PA signal. AuNR with the resonance wavelength of 1064 nm was co-cultured with the macrophages for phagocytosis. PA signal was successfully detected from macrophages with AuNR by both OR-PAM and AR-PAM. PA imaging of the macrophages with AuNR indicates inflammation in the vulnerable plaque and AR-PAM method would be applicable for clinical settings.

本文言語English
ホスト出版物のタイトル2015 37th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2015
出版社Institute of Electrical and Electronics Engineers Inc.
ページ2387-2390
ページ数4
ISBN(電子版)9781424492718
DOI
出版ステータスPublished - 2015 11 4
イベント37th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2015 - Milan, Italy
継続期間: 2015 8 252015 8 29

出版物シリーズ

名前Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS
2015-November
ISSN(印刷版)1557-170X

Other

Other37th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2015
国/地域Italy
CityMilan
Period15/8/2515/8/29

ASJC Scopus subject areas

  • 信号処理
  • 生体医工学
  • コンピュータ ビジョンおよびパターン認識
  • 健康情報学

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