Loss of heterozygosity and DNA damage repair in Saccharomyces cerevisiae

Yasukazu Daigaku, Kingo Endo, Eri Watanabe, Tetsuya Ono, Kazuo Yamamoto

研究成果: Article査読

21 被引用数 (Scopus)

抄録

Loss of heterozygosity (LOH) of tumor suppressor genes is a crucial step in the development of sporadic and hereditary cancer. Understanding how LOH events arise may provide an opportunity for the prevention or early intervention of cancer development. In an effort to investigate the source of LOH events, we constructed MATα can1Δ::LEU2 and MATa CAN1 haploid yeast strains and examined canavanine-resistance mutations in a MATa CAN1/MATα can1Δ::LEU2 heterozygote formed by mating UV-irradiated and nonirradiated haploids. An increase in LOH was observed when the irradiated CAN1 haploid was mated with nonirradiated can1Δ::LEU2, while reversed irradiation only marginally increased LOH. In the rad51Δ background, allelic crossover type LOH increased following UV irradiation but not gene conversion. In the rad52Δ background, neither type of LOH increased. The chromosome structure following LOH and the requirement for Rad51 and Rad52 proteins indicated the involvement of gene conversion, allelic crossover and break-induced replication. We argued that LOH events could have occurred during the repair of double-strand breaks on a functional (damaged) but not nonfunctional (undamaged) chromosome through recombination.

本文言語English
ページ(範囲)183-191
ページ数9
ジャーナルMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis
556
1-2
DOI
出版ステータスPublished - 2004 11月 22
外部発表はい

ASJC Scopus subject areas

  • 分子生物学
  • 遺伝学
  • 健康、毒物学および変異誘発

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