Loss of DNA binding ability of the transcription factor encoded by the mutant Mi locus

Eiichi Morii, Kimiko Takebayashi, Hozumi Motohashi, Masayuki Yamamoto, Shintaro Nomura, Yukihiko Kitamura

研究成果: Article査読

52 被引用数 (Scopus)

抄録

The mi locus of mice encodes a novel member of the basic-helix-loop-helix-leucine zipper protein family of transcription factors (MITF). Mutant mice of the mi/mi genotype have the deletion of an arginine in the basic domain and show the abnormality in development of eyes, melanocytes, osteoclasts and mast cells. The expression of the mast cell protease 6 (MMCP-6) gene is markedly reduced in mi/mi mice. We examined the in vitro binding ability of the normal and mutant MITF to a hexameric motif (CACATG) located in the 5′-flanking sequence of the MMCP-6 gene. The normal MITF bound the hexameric motif but the mutant MITF did not, suggesting that the MITF directly regulated the expression of the MMCP-6 gene.

本文言語English
ページ(範囲)1299-1304
ページ数6
ジャーナルBiochemical and biophysical research communications
205
2
DOI
出版ステータスPublished - 1994 12 15

ASJC Scopus subject areas

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学

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