Loosening of Lipid Packing Promotes Oligoarginine Entry into Cells

Tomo Murayama, Toshihiro Masuda, Sergii Afonin, Kenichi Kawano, Tomoka Takatani-Nakase, Hiroki Ida, Yasufumi Takahashi, Takeshi Fukuma, Anne S. Ulrich, Shiroh Futaki

研究成果: Article査読

38 被引用数 (Scopus)

抄録

Despite extensive use of arginine-rich cell-penetrating peptides (CPPs)—including octaarginine (R8)—as intracellular delivery vectors, mechanisms for their internalization are still under debate. Lipid packing in live cell membranes was characterized using a polarity-sensitive dye (di-4-ANEPPDHQ), and evaluated in terms of generalized polarization. Treatment with membrane curvature-inducing peptides led to significant loosening of the lipid packing, resulting in an enhanced R8 penetration. Pyrenebutyrate (PyB) is known to facilitate R8 membrane translocation by working as a hydrophobic counteranion. Interestingly, PyB also actively induced membrane curvature and perturbed lipid packing. R8 is known to directly cross cell membranes at elevated concentrations. The sites of R8 influx were found to have looser lipid packing than surrounding areas. Lipid packing loosening is proposed as a key factor that governs the membrane translocation of CPPs.

本文言語English
ページ(範囲)7644-7647
ページ数4
ジャーナルAngewandte Chemie - International Edition
56
26
DOI
出版ステータスPublished - 2017 6 19

ASJC Scopus subject areas

  • 触媒
  • 化学 (全般)

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