Longitudinal diffusion behavior of hemicyanine dyes across phospholipid vesicle membranes as studied by second-harmonic generation and fluorescence spectroscopies

Akira Yamaguchi, Masaki Nakano, Kimihisa Nochi, Tomohisa Yamashita, Kotaro Morita, Norio Teramae

研究成果: Article査読

24 被引用数 (Scopus)

抄録

The adsorption and longitudinal diffusion behaviors of a series of hemicyanine dyes to phospholipid vesicle membranes were studied by second-harmonic generation (SHG) and fluorescence spectroscopies. It was observed that the longitudinal diffusion of cationic hemicyanine dyes takes place immediately after the initial adsorption of these dyes to the outer surface of the vesicle membrane. In contrast, hardly any amount of a zwitterionic hemicyanine dye with a sulfonate group diffused across the vesicle membrane within the measurement time (<2000 s). Based on the difference in the time-course responses of SHG and fluorescence spectroscopies for all of the hemicyanine dyes tested, we propose that hydration of the sulfonate group is mainly responsible for the low diffusivity of the zwitterionic hemicyanine dye.

本文言語English
ページ(範囲)627-632
ページ数6
ジャーナルAnalytical and Bioanalytical Chemistry
386
3
DOI
出版ステータスPublished - 2006 10月
外部発表はい

ASJC Scopus subject areas

  • 分析化学
  • 生化学

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