Localization of brain-type fatty acid-binding protein in Kupffer cells of mice and its transient decrease in response to lipopolysaccharide

Soha Abdelkawi Abdelwahab, Yuji Owada, Noriko Kitanaka, Hiroo Iwasa, Hiroyuki Sakagami, Hisatake Kondo

研究成果: Article査読

25 被引用数 (Scopus)

抄録

Brain-type fatty acid-binding protein (B-FABP) was localized in Kupffer cells of liver of postnatal day 10 (P10) and older mice in immunolight and electron microscopy as well as by in situ hybridization histochemistry. The immunoreaction products were localized in the cytoplasmic matrix but not within the nucleus. After peritoneal injection of lipopolysaccharide (LPS), the immunoreaction for B-FABP decreased markedly in Kupffer cells at 1 h postinjection and thereafter gradually recovered to the preinjection level by 24 h postinjection, although no decrease in the mRNA expression was detected in Northern blotting throughout the course after the injection. The specific localization of B-FABP, but not the other FABPs, in Kupffer cells, and its rapid decrease after LPS injection suggest the intimate involvement of B-FABP in Kupffer cells in the inflammatory reaction, probably through mediation of n-3 polyunsaturated fatty acids, which are strong binders of B-FABP.

本文言語English
ページ(範囲)469-475
ページ数7
ジャーナルHistochemistry and Cell Biology
119
6
DOI
出版ステータスPublished - 2003 6 1

ASJC Scopus subject areas

  • 組織学
  • 分子生物学
  • 医療検査技術
  • 細胞生物学

フィンガープリント

「Localization of brain-type fatty acid-binding protein in Kupffer cells of mice and its transient decrease in response to lipopolysaccharide」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル