Live Cell imaging of endogenous mRNA Using RNA-Based fluorescence "turn-on" probe

Wei Qiang Ong, Y. Rose Citron, Sayaka Sekine, Bo Huang

研究成果: Article査読

26 被引用数 (Scopus)

抄録

Messenger RNA (mRNA) plays a critical role in cellular growth and development. However, there have been limited methods available to visualize endogenous mRNA in living cells with ease. We have designed RNA-based fluorescence "turn-on" probes that target mRNA by fusing an unstable form of Spinach with target-complementary sequences. These probes have been demonstrated to be selective, stable, and capable of targeting various mRNAs for live E. coli imaging.

本文言語English
ページ(範囲)200-205
ページ数6
ジャーナルACS Chemical Biology
12
1
DOI
出版ステータスPublished - 2017 1月 20
外部発表はい

ASJC Scopus subject areas

  • 生化学
  • 分子医療

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