Liposomes destabilize tRNA during heat stress

Keishi Suga, Hiroshi Umakoshi, Hibiki Tomita, Tomoyuki Tanabe, Toshinori Shimanouchi, Ryoichi Kuboi

研究成果: Article査読

11 被引用数 (Scopus)

抄録

Biomembranes play an important role in cellular response to heat stress. In this study, we focus on the interaction between liposomes and tRNA. Upon heat treatment we determined circular dichroism spectra of tRNA in presence of liposomes prepared from POPC (1-palmitoyl-2-oleoylsn-glycero-3-phosphocholine) and cholesterol (Ch). To compare thermal stability, midpoint temperature (T m) of tRNA was calculated from normalized θ208. Addition of POPC/Ch liposomes decreased the Tm value of tRNA from 48°C to 38°C. We conclude that POPC/Ch liposomes interact with tRNA and destabilize its conformation under heat stress.

本文言語English
ページ(範囲)526-529
ページ数4
ジャーナルBiotechnology Journal
5
5
DOI
出版ステータスPublished - 2010 5
外部発表はい

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Molecular Medicine

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