Lipid-tubular network formation for biochemical reaction

Shin Ichiro M. Nomura, Kazunari Akiyoshi

研究成果: Conference contribution

抄録

Here we describe liposome connecting tubular networks as a chemical reactor system. Two types of cell-sized liposomes are prepared by natural swelling method. The one was encapsulating in vitro gene-expression system (without DNA) and the other was including plasmid DNA coding green fluorescent protein (GFP). After mixing them gently, ganglioside GM3 was added for the connecting liposomes to network formation. The gene expression was checked by confocal scanning fluorescent microscopy. Fluorescence recovery after photobleaching (FRAP) assay supports the diffusive transfer could be driven the reaction. Proteoliposome network formation was also demonstrated from in vitro synthesized membrane protein cytochrome b5 on liposomes. These result should be used for constructing membrane protein array or artificial-cellular reaction cluster,

本文言語English
ホスト出版物のタイトル2007 International Symposium on Micro-NanoMechatronics and Human Science, MHS
ページ322-326
ページ数5
DOI
出版ステータスPublished - 2007
外部発表はい
イベント2007 International Symposium on Micro-NanoMechatronics and Human Science, MHS - Nagoya, Japan
継続期間: 2007 11 112007 11 14

出版物シリーズ

名前2007 International Symposium on Micro-NanoMechatronics and Human Science, MHS

Other

Other2007 International Symposium on Micro-NanoMechatronics and Human Science, MHS
国/地域Japan
CityNagoya
Period07/11/1107/11/14

ASJC Scopus subject areas

  • 人工知能
  • コンピュータ ネットワークおよび通信
  • 人間とコンピュータの相互作用
  • 電子工学および電気工学

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