Linezolid-resistant Staphylococcus aureus isolated from 2006 through 2008 at six hospitals in Japan

Yurika Ikeda-Dantsuji, Hideaki Hanaki, Fuminori Sakai, Kazunori Tomono, Yoshio Takesue, Junichi Honda, Yuriko Nonomiya, Akira Suwabe, Osanori Nagura, Katsunori Yanagihara, Hiroshige Mikamo, Kunihiko Fukuchi, Mitsuo Kaku, Shigeru Kohno, Chie Yanagisawa, Taiji Nakae, Koichiro Yoshida, Yoshihito Niki

研究成果: Article査読

30 被引用数 (Scopus)

抄録

Limited use of linezolid for treating methicillin-resistant Staphylococcus aureus (MRSA) infection was approved in Japan in 2006. We report here the status of linezolid-resistant MRSAs in Japan. Eleven linezolid-resistant clinical isolates from 11 patients at six hospitals were collected from 2006 through 2008. The minimal inhibitory concentration (MIC) of linezolid in these strains varied from 8 to 64 μg/ml. All strains had at least one G2576T mutation in the chromosomal gene(s) encoding domain V of the 23S ribosomal RNA (rRNA). Chromosomal DNA encoding five copies of the domain V region was analyzed by polymerase chain reaction (PCR). Strains with the linezolid MICs of 64, 32, 16, and 8 μg/ml had the G2576T mutation(s) in four, three (or four), two, and one copy of the 23S rRNA genes, respectively. These results suggest that the level of linezolid resistance seems to be roughly correlated with the number of mutations in the genes encoding 23S rRNA. DNA samples from all 11 strains were subjected to pulsed-field gel electrophoresis and were classified into seven independent clones having >92% identity. Among the 11 patients, five had been treated with linezolid and the remainder, in two hospitals, had no history of prior linezolid use. The results suggested possible nosocomial infections by linezolid-resistant MRSA.

本文言語English
ページ(範囲)45-51
ページ数7
ジャーナルJournal of Infection and Chemotherapy
17
1
DOI
出版ステータスPublished - 2011 2

ASJC Scopus subject areas

  • 微生物学(医療)
  • 薬理学(医学)
  • 感染症

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