Larval mesenchyme cell specification in the primitive echinoid occurs independently of the double-negative gate

Atsuko Yamazaki, Yumi Kidachi, Masaaki Yamaguchi, Takuya Minokawa

研究成果: Article査読

22 被引用数 (Scopus)

抄録

Echinoids (sea urchins) are divided into two major groups -cidaroids (a 'primitive' group) and euechinoids (a 'derived' group). The cidaroids are a promising model species for understanding the ancestral developmental mechanisms in echinoids, but little is known about the molecular mechanisms of cidaroid development. In euechinoids, skeletogenic mesenchyme cell specification is regulated by the double-negative gate (DNG), in which hesC represses the transcription of the downstream mesenchyme specification genes (alx1, tbr and ets1), thereby defining the prospective mesenchyme region. To estimate the ancestral mechanism of larval mesenchyme cell specification in echinoids, the expression patterns and roles of mesenchyme specification genes in the cidaroid Prionocidaris baculosa were examined. The present study reveals that the expression pattern and function of hesC in P. baculosa were inconsistent with the DNG model, suggesting that the euechinoid-type DNG is not utilized during cidaroid mesenchyme specification. In contrast with hesC, the expression patterns and functions of alx1, tbr and ets1 were similar between P. baculosa and euechinoids. Based on these results, we propose that the roles of alx1, tbr and ets1 in mesenchyme specification were established in the common ancestorof echinoids, and that the DNG system was acquired in the euechinoid lineage after divergence from the cidaroid ancestor. The evolutionary timing of the establishment of the DNG suggests that the DNG was originally related to micromere and/or primary mesenchyme cell formation but not to skeletogenic cell differentiation.

本文言語English
ページ(範囲)2669-2679
ページ数11
ジャーナルDevelopment (Cambridge)
141
13
DOI
出版ステータスPublished - 2014 7

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology

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