Labeling of islet cells with iron oxide nanoparticles through DNA hybridization for highly sensitive detection by MRI

Narufumi Kitamura, Ryusuke Nakai, Haruyasu Kohda, Keiko Furuta-Okamoto, Hiroo Iwata

研究成果: Article査読

20 被引用数 (Scopus)

抄録

A labeling method for islet cells with superparamagnetic iron oxide nanoparticles (SPIOs) based on DNA hybridization is proposed for monitoring of transplanted islets by magnetic resonance imaging (MRI). The surfaces of SPIOs were modified by via Michael reaction by reacting oligo-(deoxyadenylic acid)-bearing a terminal thiol group at the 5′-end ((dA)20-SH) with maleic acid functional groups on the SPIOs. The SPIOs were immobilized on islet cells which had been pretreated with oligo-(thymidylic acid)-poly(ethylene glycol)-phospholipid conjugates ((dT)20-PEG-DPPE) through DNA hybridization. Transmission electron microscopy observations revealed that SPIOs were initially anchored on the islet cell surfaces and subsequently transferred to endosomes or exfoliated with time. The SPIO-labeled islet cells could be clearly detected as dark spots by T2â̂ - -weighted MR image, whereas non-labeled islet cells could not be detected.

本文言語English
ページ(範囲)7175-7181
ページ数7
ジャーナルBioorganic and Medicinal Chemistry
21
22
DOI
出版ステータスPublished - 2013 11 15
外部発表はい

ASJC Scopus subject areas

  • 生化学
  • 分子医療
  • 分子生物学
  • 薬科学
  • 創薬
  • 臨床生化学
  • 有機化学

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