Label-free tracking of intracellular molecular crowding with cell-cycle progression using Raman microscopy

研究成果: Article査読

抄録

Intracellular environments are highly crowded with biomolecules, called molecular crowding, and the structures and functions of biomolecules in cells are influenced by molecular crowding. In this study, we tracked the changes in intracellular crowding environments during cell cycle progression using Raman imaging. The intensity ratio between the C–H and O–H stretching bands allows us to quantitatively monitor the crowding environments. The results show that the nucleus is more crowded in the M phase than in other phases; however, the cytoplasm is maintained. The changes in molecular crowding in the nucleus can affect the stability and regulation of biomolecules.

本文言語English
論文番号138843
ジャーナルChemical Physics Letters
779
DOI
出版ステータスPublished - 2021 9 16

ASJC Scopus subject areas

  • 物理学および天文学(全般)
  • 物理化学および理論化学

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