Label-free detection of DNA molecules moving in micro-fluidic channels by infrared absorption spectroscopy

Tomoyuki Miyoshi, Ken ichi Ishibashi, Koichiro Miyamoto, Ayumi Hirano-Iwata, Yasuo Kimura, Michio Niwano

研究成果: Article査読

1 被引用数 (Scopus)

抄録

Infrared absorption spectroscopy (IRAS) in a multiple internal reflection (MIR) geometry is integrated with silicon-based micro-fluidic channels to allow detection and separation of DNA molecules in the micro-channels. The micro-channels were fabricated on a Si MIR-prism using the conventional silicon lithographic techniques. In the present method we can probe solution-phase biomolecular species through IR spectral profiles of those biomolecules, allowing us to detect and separate biomolecules without fluorescence labeling. The applicability of this approach is demonstrated with a representative test case: transport of single-stranded DNA molecules (oligonucleotides) in the micro-fluidic channels.

本文言語English
ページ(範囲)917-922
ページ数6
ジャーナルSensors and Actuators, B: Chemical
238
DOI
出版ステータスPublished - 2017 1 1

ASJC Scopus subject areas

  • 電子材料、光学材料、および磁性材料
  • 器械工学
  • 凝縮系物理学
  • 表面、皮膜および薄膜
  • 金属および合金
  • 電子工学および電気工学
  • 材料化学

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