Label-free detection of DNA hybridization with light-addressable potentiometric sensors: Comparison of various DNAimmobilization strategies

T. Bronder, C. S. Wu, A. Poghossian, C. F. Werner, M. Keusgend, M. J. Schöning

研究成果: Conference article査読

11 被引用数 (Scopus)

抄録

Light-addressable potentiometric sensors (LAPS) consisting of a p-Si-SiO2 and p-Si-SiO2-Au structure, respectively, have been tested for a label-free electrical detection of DNA (deoxyribonucleic acid) hybridization. Three different strategies for immobilizing single-stranded probe DNA (ssDNA) molecules on a LAPS surface have been studied and compared: (a) immobilization of thiol-modified ssDNA on the patterned Au surface via gold-thiol bond, (b) covalent immobilization of amino-modified ssDNA onto the SiO2 surface functionalized with 3-aminopropyltriethoxysilane and (c) layer-by-layer adsorption of negatively charged ssDNA on a positively charged weak polyelectrolyte layer of poly(allylamine hydrochloride).

本文言語English
ページ(範囲)755-758
ページ数4
ジャーナルProcedia Engineering
87
DOI
出版ステータスPublished - 2014
外部発表はい
イベント28th European Conference on Solid-State Transducers, EUROSENSORS 2014 - Brescia, Italy
継続期間: 2014 9 72014 9 10

ASJC Scopus subject areas

  • 工学(全般)

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