Label-free biomedical imaging of hydrodynamics in single human cells

Hisanori Fukunaga, Hirofumi Yoshimura, Yukio Nishina, Yoji Nagashima, Masaru Tachibana

研究成果: Article査読

2 被引用数 (Scopus)

抄録

Intracellular hydrodynamics is of considerable importance to regulate cellular functions. Using Raman microspectroscopy and imaging, we visualized the time-dependent changes of water concentration in single human acute promyelocytic leukemia HL-60 cells, and also showed the labelfree biomedical imaging of water molecules, lipids and proteins for analyzing molecular distribution. Taken together, our results demonstrated that we can distinguish between intracellular water and other major cell components, and that when the physiological status of cells changes with time, the Raman spectral intensities for intracellular water concentration also changes. Therefore, this biospectroscopic analysis of intracellular hydrodynamics can provide biomarkers to examine activities in dynamic living systems, and has significant potential in determining cell specificity for novel therapeutic approaches.

本文言語English
ページ(範囲)177-181
ページ数5
ジャーナルBiomedical Research
31
3
DOI
出版ステータスPublished - 2010 6月
外部発表はい

ASJC Scopus subject areas

  • 生化学、遺伝学、分子生物学(全般)

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