Label-free and real time monitoring of adipocyte differentiation by surface infrared spectroscopy

Yuki Aonuma, Yasuhiko Kondo, Ayumi Hirano-Iwata, Atena Nishikawa, Yasuo Shinohara, Hiroo Iwata, Yasuo Kimura, Michio Niwano

研究成果: Article査読

5 被引用数 (Scopus)

抄録

We have investigated in situ real-time monitoring of cell differentiation to adipocytes by using infrared absorption spectroscopy with the multiple internal reflection (MIR-IRAS) geometry. Preadipocyte 3T3-L1 cells were cultured on a silicon prism and their adipogenesis was monitored with MIR-IRAS for seven days. Differentiated cells showed a C=O stretching band of triacylglycerides, together with the protein amide I and amide II bands. The C=O stretching band appeared before optical microscopic confirmation of fat droplet formation, a typical morphological marker for adipocytes, demonstrating that MIR-IRAS is particularly useful for non-destructive monitoring of early adipogenesis. An effect of genistein, an antiadipogenic agent, was also investigated based on the peak intensity of the C=O stretching and amide bands. Thus, MIR-IRAS has a potential to evaluate antiadipogenic agents in terms of their effects on fat and protein synthesis during adipogenesis.

本文言語English
ページ(範囲)1176-1182
ページ数7
ジャーナルSensors and Actuators, B: Chemical
176
DOI
出版ステータスPublished - 2013

ASJC Scopus subject areas

  • 電子材料、光学材料、および磁性材料
  • 器械工学
  • 凝縮系物理学
  • 表面、皮膜および薄膜
  • 金属および合金
  • 電子工学および電気工学
  • 材料化学

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