Isolation of inositol hexaphosphate (IHP)-degrading bacteria from arbuscular mycorrhizal fungal hyphal compartments using a modified baiting method involving alginate beads containing IHP

Shintaro Hara, Masanori Saito

研究成果: Article査読

3 被引用数 (Scopus)

抄録

Phytate (inositol hexaphosphate; IHP)-degrading microbes have been suggested to contribute to arbuscular mycorrhizal fungi (AMF)-mediated P transfer from IHP to plants; however, no IHP degrader involved in AMF-mediated P transfer has been isolated to date. We herein report the isolation of IHP-degrading bacteria using a modified baiting method. We applied alginate beads as carriers of IHP powder, and used them as recoverable IHP in the AM fungal compartment of plant cultivation experiments. P transfer from IHP in alginate beads via AMF was confirmed, and extracted DNA from alginate beads was analyzed by denaturing gradient gel electrophoresis targeting the 16S rRNA gene and a clone library method for the beta-propeller phytase (BPP) gene. The diversities of the 16S rRNA and BPP genes of microbes growing on IHP beads were simple and those of Sphingomonas spp. and Caulobacter spp. dominated. A total of 187 IHP-utilizing bacteria were isolated and identified, and they were consistent with the results of DNA analysis. Furthermore, some isolated Sphingomonas spp. and Caulobacter sp. showed IHP-degrading activity. Therefore, we successfully isolated dominant IHP-degrading bacteria from IHP in an AMF hyphal compartment. These strains may contribute to P transfer from IHP via AMF.

本文言語English
ページ(範囲)234-243
ページ数10
ジャーナルMicrobes and environments
31
3
DOI
出版ステータスPublished - 2016

ASJC Scopus subject areas

  • 生態、進化、行動および分類学
  • 土壌科学
  • 植物科学

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