Isolation of genes for low-temperature-induced proteins in rice by a simple subtractive method

Kripamoy Aguan, Kazuyuki Sugawara, Nobuhiro Suzuki, Tomonobu Kusano

    研究成果: Article査読

    48 被引用数 (Scopus)

    抄録

    We have isolated cDNAs that correspond to three distinct low-temperature-induced mRNAs from rice cells using an effective, simple subtraction method. The corresponding genes were designated lip (gene encoding low-temperature-induced protein) -5,-9 and -19. The expression of these genes was slightly stimulated lip5 and lip19) or unaffected (lip9) by abscisic acid, unlike the expression of the rabl6A-D genes which are readily induced by either abscisic acid or high osmotic stress. Time-course analysis revealed that while the lip5 gene was induced 3 h after temperature down-shift, and the lip9 and lipl9 genes were induced 6 h after such a shift.

    本文言語English
    ページ(範囲)1285-1289
    ページ数5
    ジャーナルPlant and Cell Physiology
    32
    8
    出版ステータスPublished - 1991 12

    ASJC Scopus subject areas

    • 生理学
    • 植物科学
    • 細胞生物学

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