Isolation of acini from nasal glands of the guinea‐pig

H. SUNOSE, W. ZHANG, M. ISHIGAKI, Y. KATORI, M. SUZUKI, K. IKEDA, T. TAKASAKA, Y. SAITO, A. NISHIYAMA

研究成果: Article査読

11 被引用数 (Scopus)

抄録

A procedure for isolating the acinar cells of the serous gland in the mammalian nasal septum has been developed. This technique is characterized by meticulous and selective isolation with minimal contamination by the surface epithelial cells and employs enzymatic treatment with collagenase. The isolated cells were confirmed to be serous gland acini as shown by negative staining with Alcian blue and a high electron density of the granules. The acini were more than 90% viable as judged by trypan blue exclusion. Ultrastructural integrity of the cells was well maintained following the isolation procedure. Application of acetylcholine to the isolated acini induced an inward current in a whole‐cell patch clamp and increased intracellular Ca2+ concentration measured by fura‐2. These acetylcholine responses were completely blocked by atropine. These physiological findings directly demonstrated that nasal gland acini possess muscarinic‐activated receptors as previously suggested. These isolated cells hold promise for the in vitro study of secretory mechanisms in the mammalian nasal gland.

本文言語English
ページ(範囲)377-384
ページ数8
ジャーナルActa Physiologica Scandinavica
151
3
DOI
出版ステータスPublished - 1994 7月

ASJC Scopus subject areas

  • 生理学

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