Isolation of a vascular cell wall-specific monoclonal antibody recognizing a cell polarity by using a phage display subtraction method

Naoki Shinohara, Taku Demura, Hiroo Fukuda

    研究成果: Article査読

    31 被引用数 (Scopus)

    抄録

    Using a strategy consisting of (i) the isolation of cell walls from synchronously differentiating cells of Zinnia, (ii) the generation of mAbs with an antibody phage display method, and (iii) screening with a subtraction method, we isolated mAbs recognizing vascular development-specific cell wall components without prior antigen identification. One of the isolated mAbs, designated CN 8, recognized a cell wall component contained in the hemicellulosic fraction. Immunohistochemical analyses showed that the CN 8 epitope was localized to the cell wall of immature treachery elements and xylem parenchyma cells. In immature treachery elements, the CN 8 epitope had a polarized localization pattern regardless of whether the cells are formed as parts of vessels in situ or as single treachery elements in vitro, suggesting that cell polarity autonomously formed on the cell wall may function in treachery element differentiation.

    本文言語English
    ページ(範囲)2585-2590
    ページ数6
    ジャーナルProceedings of the National Academy of Sciences of the United States of America
    97
    6
    DOI
    出版ステータスPublished - 2000 3 14

    ASJC Scopus subject areas

    • General

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