Isolation of a cDNA for a nucleoside diphosphate kinase capable of phosphorylating the kinase domain of the self-incompatibility factor SRK of Brassica campestris

Yasuhiko Matsushita, Tatsuya Suzuki, Ryo Kubota, Masako Mori, Hiroko Shimosato, Masao Watanabe, Toshiaki Kayano, Takeshi Nishio, Hiroshi Nyunoya

研究成果: Article査読

9 被引用数 (Scopus)

抄録

SRK is a plant receptor kinase involved in the self-incompatibility system of Brassica species. During a cDNA screening for the phosphoproteins from a stigma expression library, a clone encoding the nucleoside diphosphate kinase III (Bc-NDPK III) was obtained. After in vitro phosphorylation assays with recombinant proteins, Bc-NDPK III contained mostly phosphoserine. By contrast, the kinase domain of SRK contained phosphoserine and phosphothreonine, both of which were significantly increased by the addition of Bc-NDPK III in the presence of an SRK inhibitor KN-62. The result suggested the possible involvement of Bc-NDPK III in the signal transduction pathway through SRK.

本文言語English
ページ(範囲)765-767
ページ数3
ジャーナルJournal of experimental botany
53
369
DOI
出版ステータスPublished - 2002
外部発表はい

ASJC Scopus subject areas

  • 生理学
  • 植物科学

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