Isolation and properties of an S-adenosyl-L-methionine binding protein from the green alga, Chlamydomonas reinhardi

Y. Nakano, N. Koizumi, T. Kusano, H. Sano

    研究成果: Article査読

    3 被引用数 (Scopus)

    抄録

    A protein that binds S-adenosyl-L-methionine (AdoMet) was isolated by successive chromatography from vegetative cells of Chlamydomonas reinhardi. The relative molecular masses of native and denatured proteins were calculated to be 15 kDa and 16 kDa, respectively. The reaction was specific to AdoMet and reversible as shown by competition experiments. The optimal pH for binding ranged between 7.5 and 9.0, and the apparent Ka for AdoMet was 1.0 μmol/L. Results suggested that the protein may function as the carrier to stabilize AdoMet, which is otherwise labile in the cellular environment.

    本文言語English
    ページ(範囲)707-711
    ページ数5
    ジャーナルJournal of Plant Physiology
    157
    6
    DOI
    出版ステータスPublished - 2000

    ASJC Scopus subject areas

    • 生理学
    • 農業および作物学
    • 植物科学

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