Isolation and characterization of the active cDNA of the human cell cycle gene (RCC1) involved in the regulation of onset of chromosome condensation.

M. Ohtsubo, R. Kai, N. Furuno, T. Sekiguchi, M. Sekiguchi, H. Hayashida, K. Kuma, T. Miyata, S. Fukushige, T. Murotsu

研究成果: Article査読

150 被引用数 (Scopus)

抄録

The human RCC1 gene was cloned after DNA-mediated gene transfer into the tsBN2 cell line, which shows premature chromosome condensation at nonpermissive temperatures (39.5-40 degrees C). This gene codes for a 2.5-kb poly(A)+ RNA that is well conserved in hamsters and humans. We isolated 15 cDNA clones from the Okayama-Berg human cDNA library, and found two that can complement the tsBN2 mutation with an efficiency comparable to that of the genomic DNA clone. The base sequences of these two active cDNA clones differ at the 5' proximal end, yet both have a common open reading frame, encoding a protein of 421 amino acids with a calculated molecular weight of 44,847 and with seven homologous repeated domains of about 60 amino acids. This human RCC1 gene was located to human chromosome 1 using sorted chromosomal fractions.

本文言語English
ページ(範囲)585-593
ページ数9
ジャーナルGenes & development
1
6
DOI
出版ステータスPublished - 1987 8
外部発表はい

ASJC Scopus subject areas

  • 遺伝学
  • 発生生物学

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