Isolation and characterization of human reg protein produced in Saccharomyces cerevisiae

Takako Itoh, Hiroshige Tsuzuki, Takaaki Katoh, Hiroshi Teraoka, Kohichi Matsumoto, Nobuo Yoshida, Kimio Terazono, Takuo Watanabe, Hideto Yonekura, Hiroshi Yamamoto, Hiroshi Okamoto

研究成果: Article査読

18 被引用数 (Scopus)

抄録

reg was originally identified a a gene expressed during the regeneration of insulin-producing pancreatic β-cells of the rat. We built an expression vector containing human reg cDNA to drive Saccharomyces cerevisiae to synthesize the reg protein, and purified it from the culture medium. The 144-amino acid sequence of the recombinant protein was consistent with that deduced from the cDNA and genomic DNA sequence except that the signal sequence of 22 amino acids was eliminated, and the amino-terminal residue of the protein was pyroglutamic acid. The secondary structure of the reg protein was predicted by determination of the intramolecular cystine linkage and of α-helix and β-sheet contents.

本文言語English
ページ(範囲)85-88
ページ数4
ジャーナルFEBS Letters
272
1-2
DOI
出版ステータスPublished - 1990 10 15

ASJC Scopus subject areas

  • 生物理学
  • 構造生物学
  • 生化学
  • 分子生物学
  • 遺伝学
  • 細胞生物学

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