Irradiation inhibits vascular anastomotic stenosis in a canine model

Takeshi Saito, Atsushi Iguchi, Koichi Tabayashi

研究成果: Article査読

7 被引用数 (Scopus)


Objective. The graft patency rate after coronary artery bypass grafting (CABG) correlates with anastomotic stenosis. Intracoronary radiation therapy is effective for preventing restenosis after percutaneous coronary intervention (PCI). We postulated that intracoronary radiation therapy could prevent anastomotic stenosis and tested this hypothesis in an animal model. Methods. Femoral arteries and veins of beagle dogs were harvested, and composite arterioarterial and arteriovenous grafts were prepared. After external irradiation of the anastomotic sites, these composite grafts were transplanted into femoral arteries. Histomorphometric and immunohistological analyses of the anastomotic sites were performed. The study groups consisted of controls and animals exposed to 10 Gy, 20 Gy, and 30 Gy (n = 5, in each group). Results. In the artery graft model, the ratio of negative remodeling was significantly increased in all groups exposed to >10 Gy. The ratio of neointimal hyperplasia was significantly decreased in all groups exposed to >10 Gy. Cell density of anti-α-actin antibody-positive cells and anti-proliferating cell nuclear antigen (PCNA) antibody-positive cells was highest in the adventitial layer, and the density decreased as the dosage increased. Experimental results were almost the same in the vein graft models as in the artery graft models. With double immunohistostaining, the anti-PCNA antibody-positive cells expressed α-actin. Conclusion. Irradiation can inhibit anastomotic stenosis in a canine model. Adventitia is a factor in the creation of stenosis, and irradiation appears to target the adventitia. We speculate that there might be a possible role for intracoronary irradiation in the future to prevent anas-tomotic stenosis.

ジャーナルGeneral thoracic and cardiovascular surgery
出版ステータスPublished - 2009 8月

ASJC Scopus subject areas

  • 外科
  • 呼吸器内科
  • 循環器および心血管医学


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