Ionic liquid/water interfacial localization of a green fluorescent protein fused to a tryptophan-rich peptide

Takashi Matsuyama, Kim Domyoung, Mitsuo Umetsu, Taiji Ikawa, Mamoru Yamanishi, Nobuhiro Ishida, Izumi Kumagai, Haruo Takahashi

研究成果: Article査読

5 被引用数 (Scopus)

抄録

We report that several tryptophan-rich peptides exhibit an affinity for a hydrophobic ionic liquid (IL) (1-ethyl-3-methylimidazolium bis-trifluoromethanesulfonyl imide), and that green fluorescent protein (GFP) fused to a peptides, "SSSWWSWWWW" (SW1) or "SWWWWSWWWW" (SW2), containing serine (S) and tryptophan (W) at the C terminus localized at the IL/water interface. While GFPs without W-rich peptide distributed only in water phase, SW1- and SW2-GFPs were accumulated at the interface. The localization of SW1-GFP showed biphasic behavior, and most distinctive localization was observed at 7.1. μM. The localization of SW2-GFP presumably occurred at largely lower concentration (≤ 0.5 μM) than that of SW1-GFP, which difference was due to the higher hydrophobicity of SW2 peptide.

本文言語English
ページ(範囲)160-165
ページ数6
ジャーナルJournal of Bioscience and Bioengineering
113
2
DOI
出版ステータスPublished - 2012 2

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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