Involvement of Kupffer cells in lipopolysaccharide-induced rapid accumulation of platelets in the liver and the ensuing anaphylaxis-like shock in mice

Kouji Yamaguchi, Zhiqian Yu, Hiroyuki Kumamoto, Yumiko Sugawara, Hiroshi Kawamura, Haruhiko Takada, Takashi Yokochi, Shunji Sugawara, Yasuo Endo

研究成果: Article査読

22 被引用数 (Scopus)

抄録

Intravenous injection of Klebsiella O3 lipopolysaccharide (LPS) into BALB/c mice induces an anaphylaxis-like shock within minutes. Using 5-hydroxytryptamine as a marker for platelets, we previously suggested that a rapid platelet accumulation in the liver and lung precedes the shock, and that a complement-dependent platelet-degradation is involved in the shock. Here, we examined (i) the effect of platelet-depletion (using an anti-platelet monoclonal antibody) on the shock and (ii) the contribution of macrophages to the platelet-accumulation in those organs. LPS-induced platelet-accumulations in the liver and lung were confirmed by immunostaining. In platelet-depleted mice, the shock was largely prevented. The number of F4/80-positive macrophages was much greater in liver than in lung, and the hepatic macrophages were largely lost in mice given clodronate-encapsulated liposomes. In mice treated with such liposomes, both the LPS-induced accumulation of platelets in the liver (but not in the lung) and the shock were largely prevented, and repopulation of hepatic macrophages restored these LPS-induced responses. These results suggest that (i) platelets are indeed involved in the shock, (ii) Kupffer cells mediate the hepatic platelet accumulation, and (iii) preventing this hepatic accumulation can largely prevent rapid shock being induced by LPS (at the dose used here).

本文言語English
ページ(範囲)269-275
ページ数7
ジャーナルBiochimica et Biophysica Acta - Molecular Basis of Disease
1762
3
DOI
出版ステータスPublished - 2006 3

ASJC Scopus subject areas

  • Molecular Medicine
  • Molecular Biology

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