Investigation of biocompatibility on nitrogen-doped a-C:H film coating scaffold surface in in-vivo and in-vitro tests

Yasuharu Ohgoe, Tomoaki Wada, Yasuyuki Shiraishi, Hidekazu Miura, Kenji K. Hirakuri, Akio Funakubo, Tomoyuki Yambe, Yasuhiro Fukui

研究成果: Conference contribution

2 被引用数 (Scopus)


In this study, in order to investigate biocompatibility of nitrogen-doped hydrogenated amorphous carbon (a-C:H:N) film coating segmented polyurethane (SPU) scaffold fiber sheet (a-C:H:N-Scaffold) in in-vitro test, mouse fibroblasts (NIH 3T3) cells were grown on the a-C:H:N-Scaffold. The cell behavior was monitored by time-lapse imaging system. Additionally, the a-C:H:N-Scaffold was implanted at partial aorta descendens of a goat for 35 days. The surface morphology, composition, and wettability of the a-C:H:N-scaffold was estimated by Scanning Electron Microscope (SEM), X-ray photoelectron spectrometer (XPS), and contact angle measurement. In in-vitro test, it was observed that a-C:H:N film coating had a facilitatory effect on cell motility and cell growth. In in-vivo test, it was observed that the a-C:H:N-Scaffold surface was uniformly covered by neointima. The a-C:H:N-Scaffold surface had no thrombus formation as an inflammatory reaction and it was shown that the a-C:H:N film coating had a good blood compatibility. These results suggest that a-C:H:N film coating has good cytocompatibility and blood compatibility and it is a promising approach for improvement of biocompatibility of biomaterial surfaces.

ホスト出版物のタイトルBiomimetic, Bio-Inspired and Self-Assembled Materials for Engineered Surfaces and Applications
出版ステータスPublished - 2013
イベント2012 MRS Fall Meeting - Boston, MA, United States
継続期間: 2012 11月 252012 11月 30


名前Materials Research Society Symposium Proceedings


Other2012 MRS Fall Meeting
国/地域United States
CityBoston, MA

ASJC Scopus subject areas

  • 材料科学(全般)
  • 凝縮系物理学
  • 材料力学
  • 機械工学


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