Invariant natural killer T cells recognize glycolipids from pathogenic Gram-positive bacteria

Yuki Kinjo; Petr Illarionov; José Luis Vela; Bo Pei; Enrico Girardi; Xiangming Li; Yali Li; Masakazu Imamura; Yukihiro Kaneko; Akiko Okawara; Yoshitsugu Miyazaki; Anaximandro Gómez-Velasco; Paul Rogers; Samira Dahesh; Satoshi Uchiyama; Archana Khurana; Kazuyoshi Kawahara; Hasan Yesilkaya; Peter W. Andrew; Chi Huey Wong; Kazuyoshi Kawakami; Victor Nizet; Gurdyal S. Besra; Moriya Tsuji; Dirk M. Zajonc; Mitchell Kronenberg

doi:10.1038/ni.2096

Invariant natural killer T cells recognize glycolipids from pathogenic Gram-positive bacteria

Yuki Kinjo, Petr Illarionov, José Luis Vela, Bo Pei, Enrico Girardi, Xiangming Li, Yali Li, Masakazu Imamura, Yukihiro Kaneko, Akiko Okawara, Yoshitsugu Miyazaki, Anaximandro Gómez-Velasco, Paul Rogers, Samira Dahesh, Satoshi Uchiyama, Archana Khurana, Kazuyoshi Kawahara, Hasan Yesilkaya, Peter W. Andrew, Chi Huey WongKazuyoshi Kawakami, Victor Nizet, Gurdyal S. Besra, Moriya Tsuji, Dirk M. Zajonc, Mitchell Kronenberg

医学系研究科・保健学専攻

研究成果: Article › 査読

270 被引用数 (Scopus)

抄録

Natural killer T cells (NKT cells) recognize glycolipid antigens presented by CD1d. These cells express an evolutionarily conserved, invariant T cell antigen receptor (TCR), but the forces that drive TCR conservation have remained uncertain. Here we show that NKT cells recognized diacylglycerol-containing glycolipids from Streptococcus pneumoniae, the leading cause of community-acquired pneumonia, and group B Streptococcus, which causes neonatal sepsis and meningitis. Furthermore, CD1d-dependent responses by NKT cells were required for activation and host protection. The glycolipid response was dependent on vaccenic acid, which is present in low concentrations in mammalian cells. Our results show how microbial lipids position the sugar for recognition by the invariant TCR and, most notably, extend the range of microbes recognized by this conserved TCR to several clinically important bacteria.

本文言語	English
ページ（範囲）	966-974
ページ数	9
ジャーナル	Nature Immunology
巻	12
号	10
DOI	https://doi.org/10.1038/ni.2096
出版ステータス	Published - 2011 10月

ASJC Scopus subject areas

免疫アレルギー学
免疫学

文献へのアクセス

10.1038/ni.2096

他のファイルとリンク

引用スタイル

Kinjo, Y., Illarionov, P., Vela, J. L., Pei, B., Girardi, E., Li, X., Li, Y., Imamura, M., Kaneko, Y., Okawara, A., Miyazaki, Y., Gómez-Velasco, A., Rogers, P., Dahesh, S., Uchiyama, S., Khurana, A., Kawahara, K., Yesilkaya, H., Andrew, P. W., ... Kronenberg, M. (2011). Invariant natural killer T cells recognize glycolipids from pathogenic Gram-positive bacteria. Nature Immunology, 12(10), 966-974. https://doi.org/10.1038/ni.2096

Kinjo, Y, Illarionov, P, Vela, JL, Pei, B, Girardi, E, Li, X, Li, Y, Imamura, M, Kaneko, Y, Okawara, A, Miyazaki, Y, Gómez-Velasco, A, Rogers, P, Dahesh, S, Uchiyama, S, Khurana, A, Kawahara, K, Yesilkaya, H, Andrew, PW, Wong, CH, Kawakami, K, Nizet, V, Besra, GS, Tsuji, M, Zajonc, DM & Kronenberg, M 2011, 'Invariant natural killer T cells recognize glycolipids from pathogenic Gram-positive bacteria', Nature Immunology, vol. 12, no. 10, pp. 966-974. https://doi.org/10.1038/ni.2096

@article{87b2abe1349c4802ab2adb998fbc82b2,

title = "Invariant natural killer T cells recognize glycolipids from pathogenic Gram-positive bacteria",

abstract = "Natural killer T cells (NKT cells) recognize glycolipid antigens presented by CD1d. These cells express an evolutionarily conserved, invariant T cell antigen receptor (TCR), but the forces that drive TCR conservation have remained uncertain. Here we show that NKT cells recognized diacylglycerol-containing glycolipids from Streptococcus pneumoniae, the leading cause of community-acquired pneumonia, and group B Streptococcus, which causes neonatal sepsis and meningitis. Furthermore, CD1d-dependent responses by NKT cells were required for activation and host protection. The glycolipid response was dependent on vaccenic acid, which is present in low concentrations in mammalian cells. Our results show how microbial lipids position the sugar for recognition by the invariant TCR and, most notably, extend the range of microbes recognized by this conserved TCR to several clinically important bacteria.",

author = "Yuki Kinjo and Petr Illarionov and Vela, {Jos{\'e} Luis} and Bo Pei and Enrico Girardi and Xiangming Li and Yali Li and Masakazu Imamura and Yukihiro Kaneko and Akiko Okawara and Yoshitsugu Miyazaki and Anaximandro G{\'o}mez-Velasco and Paul Rogers and Samira Dahesh and Satoshi Uchiyama and Archana Khurana and Kazuyoshi Kawahara and Hasan Yesilkaya and Andrew, {Peter W.} and Wong, {Chi Huey} and Kazuyoshi Kawakami and Victor Nizet and Besra, {Gurdyal S.} and Moriya Tsuji and Zajonc, {Dirk M.} and Mitchell Kronenberg",

note = "Funding Information: We thank S. Akira (Osaka University) for Myd88−/− mice; B. Beutler (Scripps Research Institute) for Myd88−/−TrifLps2/Lps2 mice; M. Antony (University of Birmingham) for group B Streptococcus A909; N. Yamamoto, T. Kinjo, G.D. Ainge, D. Gibson and G. Painter for suggestions; N. Sato for help with glycolipid analysis; and C. Lena for technical assistance. Supported by the US National Institutes of Health (AI45053 and AI71922 to M.K., AI074952 to D.M.Z.; AI070258 to M.T.; and F32AI083029 to J.L.V.), the Japan Society for the Promotion of Science and Ministry of Education, Culture, Sports, Science and Technology (22689031), the Ministry of Health, Labor and Welfare of Japan (H22seisakusouyakuippan012), the Uehara Memorial Foundation (Y.Ki.), the Wellcome Trust, the Royal Society, J. Bardrick (G.S.B.) and the Cancer Research Institute (D.M.Z.).",

year = "2011",

month = oct,

doi = "10.1038/ni.2096",

language = "English",

volume = "12",

pages = "966--974",

journal = "Nature Immunology",

issn = "1529-2908",

publisher = "Nature Publishing Group",

number = "10",

}

TY - JOUR

T1 - Invariant natural killer T cells recognize glycolipids from pathogenic Gram-positive bacteria

AU - Kinjo, Yuki

AU - Illarionov, Petr

AU - Vela, José Luis

AU - Pei, Bo

AU - Girardi, Enrico

AU - Li, Xiangming

AU - Li, Yali

AU - Imamura, Masakazu

AU - Kaneko, Yukihiro

AU - Okawara, Akiko

AU - Miyazaki, Yoshitsugu

AU - Gómez-Velasco, Anaximandro

AU - Rogers, Paul

AU - Dahesh, Samira

AU - Uchiyama, Satoshi

AU - Khurana, Archana

AU - Kawahara, Kazuyoshi

AU - Yesilkaya, Hasan

AU - Andrew, Peter W.

AU - Wong, Chi Huey

AU - Kawakami, Kazuyoshi

AU - Nizet, Victor

AU - Besra, Gurdyal S.

AU - Tsuji, Moriya

AU - Zajonc, Dirk M.

AU - Kronenberg, Mitchell

N1 - Funding Information: We thank S. Akira (Osaka University) for Myd88−/− mice; B. Beutler (Scripps Research Institute) for Myd88−/−TrifLps2/Lps2 mice; M. Antony (University of Birmingham) for group B Streptococcus A909; N. Yamamoto, T. Kinjo, G.D. Ainge, D. Gibson and G. Painter for suggestions; N. Sato for help with glycolipid analysis; and C. Lena for technical assistance. Supported by the US National Institutes of Health (AI45053 and AI71922 to M.K., AI074952 to D.M.Z.; AI070258 to M.T.; and F32AI083029 to J.L.V.), the Japan Society for the Promotion of Science and Ministry of Education, Culture, Sports, Science and Technology (22689031), the Ministry of Health, Labor and Welfare of Japan (H22seisakusouyakuippan012), the Uehara Memorial Foundation (Y.Ki.), the Wellcome Trust, the Royal Society, J. Bardrick (G.S.B.) and the Cancer Research Institute (D.M.Z.).

PY - 2011/10

Y1 - 2011/10

N2 - Natural killer T cells (NKT cells) recognize glycolipid antigens presented by CD1d. These cells express an evolutionarily conserved, invariant T cell antigen receptor (TCR), but the forces that drive TCR conservation have remained uncertain. Here we show that NKT cells recognized diacylglycerol-containing glycolipids from Streptococcus pneumoniae, the leading cause of community-acquired pneumonia, and group B Streptococcus, which causes neonatal sepsis and meningitis. Furthermore, CD1d-dependent responses by NKT cells were required for activation and host protection. The glycolipid response was dependent on vaccenic acid, which is present in low concentrations in mammalian cells. Our results show how microbial lipids position the sugar for recognition by the invariant TCR and, most notably, extend the range of microbes recognized by this conserved TCR to several clinically important bacteria.

AB - Natural killer T cells (NKT cells) recognize glycolipid antigens presented by CD1d. These cells express an evolutionarily conserved, invariant T cell antigen receptor (TCR), but the forces that drive TCR conservation have remained uncertain. Here we show that NKT cells recognized diacylglycerol-containing glycolipids from Streptococcus pneumoniae, the leading cause of community-acquired pneumonia, and group B Streptococcus, which causes neonatal sepsis and meningitis. Furthermore, CD1d-dependent responses by NKT cells were required for activation and host protection. The glycolipid response was dependent on vaccenic acid, which is present in low concentrations in mammalian cells. Our results show how microbial lipids position the sugar for recognition by the invariant TCR and, most notably, extend the range of microbes recognized by this conserved TCR to several clinically important bacteria.

UR - http://www.scopus.com/inward/record.url?scp=80052966551&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=80052966551&partnerID=8YFLogxK

U2 - 10.1038/ni.2096

DO - 10.1038/ni.2096

M3 - Article

C2 - 21892173

AN - SCOPUS:80052966551

SN - 1529-2908

VL - 12

SP - 966

EP - 974

JO - Nature Immunology

JF - Nature Immunology

IS - 10

ER -

Invariant natural killer T cells recognize glycolipids from pathogenic Gram-positive bacteria

抄録

ASJC Scopus subject areas

文献へのアクセス

他のファイルとリンク

フィンガープリント

引用スタイル