Intramolecular interaction of SUR2 subtypes for intracellular ADP-induced differential control of KATP channels

Kenji Matsushita, Kengo Kinoshita, Tetsuro Matsuoka, Akikazu Fujita, Takashi Fujikado, Yasuo Tano, Haruki Nakamura, Yoshihisa Kurachi

研究成果: Article査読

36 被引用数 (Scopus)

抄録

ATP-sensitive K+ (KATP) channels are composed of sulfonylurea receptors (SURs) and inwardly rectifying Kir6.2-channels. The C-terminal 42 amino acid residues (C42) of SURs are responsible for ADP-induced differential activation of KATP channels in SUR-subtypes. By examining ADP-effect on KATP channels containing various chimeras of SUR2A and SUR2B, we identified a segment of 7 residues at central portion of C42 critical for this phenomenon. A 3-D structure model of the region containing the second nucleotide-binding domain (NBD2) of SUR and C42 was developed based on the structure of HisP, a nucleotide-binding protein forming the bacterial Histidine transporter complex. In the model, the polar and charged residues in the critical segment located within a distance that allows their electrostatic interaction with Arg1344 at the Walker-A loop of NBD2. Therefore, the interaction might be involved in the control of ADP-induced differential activation of SUR2-subtype KATP channels.

本文言語English
ページ(範囲)554-561
ページ数8
ジャーナルCirculation research
90
5
DOI
出版ステータスPublished - 2002 3 22
外部発表はい

ASJC Scopus subject areas

  • 生理学
  • 循環器および心血管医学

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