Intramanchette transport during primate spermiogenesis: Expression of dynein, myosin Va, motor recruiter myosin Va, VIIa-Rab27a/b interacting protein, and Rab27b in the manchette during human and monkey spermiogenesis

Shinichi Hayasaka, Yukihiro Terada, Kichiya Suzuki, Haruo Murakawa, Ikuo Tachibana, Tadashi Sankai, Takashi Murakami, Nobuo Yaegashi, Kunihiro Okamura

研究成果: Article査読

32 被引用数 (Scopus)

抄録

Aim: To show whether molecular motor dynein on a microtubule track, molecular motor myosin Va, motor recruiter myosin Va, VIIa-Rab27a/b interacting protein (MyRIP), and vesicle receptor Rab27b on an F-actin track were present during human and monkey spermiogenesis involving intramanchette transport (IMT). Methods: Spermiogenic cells were obtained from three men with obstructive azoospermia and normal adult cynomolgus monkey (Macaca fascicularis). Immunocytochemical detection and reverse transcription-polymerase chain reaction (RT-PCR) analysis of the proteins were carried out. Samples were analyzed by light microscope. Results: Using RT-PCR, we found that dynein, myosin Va, MyRIP and Rab27b were expressed in monkey testis. These proteins were localized to the manchette, as shown by immunofluorescence, particularly during human and monkey spermiogenesis. Conclusion: We speculate that during primate spermiogenesis, those proteins that compose microtubule-based and actin-based vesicle transport systems are actually present in the manchette and might possibly be involved in intramanchette transport.

本文言語English
ページ(範囲)561-568
ページ数8
ジャーナルAsian Journal of Andrology
10
4
DOI
出版ステータスPublished - 2008 7

ASJC Scopus subject areas

  • 泌尿器学

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