Intracellular signaling similarity reveals neural stem cell-like properties of ependymal cells in the adult rat spinal cord

Masaaki Kitada, Shohei Wakao, Mari Dezawa

研究成果: Article査読

2 被引用数 (Scopus)

抄録

Proliferation of ependymal cells of the adult spinal cord (SCEp cells) in the intact condition has been considered as a quite rare event. To visualize proliferating/proliferated SCEp cells, we used the intensive 5-bromo-2′-deoxyuridine (BrdU) administration method to find that about two cells in the ependymal layer incorporated BrdU in a 10-μm-thick section. Because these two cells were not considered to undergo further proliferation, we analyzed the positioning and motility of two neighboring BrdU-incorporated proliferated cells and elucidated the tendency of the movement of SCEp cells to the outer side inside the ependymal layer. Even if it was rare, one of the proliferated cells in the ependymal layer differentiated into astrocytes. Gene introduction of Notch intracellular domain (NICD), a constitutively active form of Notch1, into SCEp cells demonstrated both increase in proliferation and induction of differentiation into astrocytes. Overexpression of Sox2 promoted proliferation in SCEp cells. The reaction of gene introduction of NICD and Sox2 indicates the similarity of intracellular signaling between SCEp cells and neural stem cells. Also, considering the fact that SCEp cells express these two factors in the intact condition, Notch and Sox2 are important for the cell fate control of SCEp cells in the intact condition.

本文言語English
ページ(範囲)326-340
ページ数15
ジャーナルDevelopment Growth and Differentiation
60
6
DOI
出版ステータスPublished - 2018 8

ASJC Scopus subject areas

  • 発生生物学
  • 細胞生物学

フィンガープリント

「Intracellular signaling similarity reveals neural stem cell-like properties of ependymal cells in the adult rat spinal cord」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル