Interleukin-3 in Cooperation with Transforming Growth Factor β Induces Granulocyte Macrophage Colony Stimulating Factor Independent Differentiation of Human CD34+ Hematopoietic Progenitor Cells into Dendritic Cells with Features of Langerhans Cells

Zia U.A. Mollah, Setsuya Aiba, Satoshi Nakagawa, Masato Mizuashi, Tomoyuki Ohtani, Yumiko Yoshino, Hachiro Tagami

研究成果: Article査読

13 被引用数 (Scopus)

抄録

Recently, we have reported that M-CSF in cooperation with TGF-β1 can induce Langerhans cell (LC) development from hematopoietic progenitor cells (HPCs) without GM-CSF. In the present study, we examined whether TGF-β1 changes the differentiation of HPCs induced by IL-3 towards LC development. We cultured HPCs in a serum-free medium in the presence of IL-3 and a combination cytokines including Flt3L, SCF, and TNF-α with or without TGF-β1. DCs induced by the IL-3 culture (IL-3 DCs) did not significantly differ from those induced by the GM-CSF culture (GM-CSF DCs). Namely, both expressed CDla, F-cadherin, and Langerin in the presence of TGF-β1 and stimulated allogeneic T cells at a similar magnitude. In contrast to GM-CSF DCs, IL-3 DCs lacked the expression of Birbeck granules (BGs) in spite of their expression of Langerin. When we compared the expression of Langerin between these two DCs, however, it became clear that both Langerin protein and mRNA were significantly lower in IL-3 DCs than in GM-CSF DCs. These studies again demonstrated the ability of TGF-β1 to polarize the differentiation of HPCs induced by IL-3 towards LC development, although IL-3 DCs were unable to form BGs partly because of their poor ability to induce Langerin.

本文言語English
ページ(範囲)1397-1401
ページ数5
ジャーナルJournal of Investigative Dermatology
121
6
DOI
出版ステータスPublished - 2003 12月

ASJC Scopus subject areas

  • 生化学
  • 分子生物学
  • 皮膚病学
  • 細胞生物学

フィンガープリント

「Interleukin-3 in Cooperation with Transforming Growth Factor β Induces Granulocyte Macrophage Colony Stimulating Factor Independent Differentiation of Human CD34+ Hematopoietic Progenitor Cells into Dendritic Cells with Features of Langerhans Cells」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

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