TY - JOUR
T1 - Interleukin-1 beta rather than lymphotoxin as the major bone resorbing activity in human multiple myeloma
AU - Kawano, M.
AU - Yamamoto, I.
AU - Iwato, K.
AU - Tanaka, H.
AU - Asaoku, H.
AU - Tanabe, O.
AU - Ishikawa, H.
AU - Nobuyoshi, M.
AU - Ohmoto, Y.
AU - Hirai, Y.
AU - Kuramoto, A.
N1 - Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.
PY - 1989
Y1 - 1989
N2 - Human myeloma cells were purified from bone marrow aspirates from four patients having advanced myeloma, including one with common acute lymphoblastic leukemia antigen-positive myeloma. All of these myelomas had marked bone lytic lesions. From the culture supernatants of these purified myeloma cells, bone-resorbing activities were significantly revealed by 45Ca-release bone resorption assay, and IL-1 activities were also detected by IL-1 bioassay (mouse thymocyte comitogenic assay). Sandwich enzyme immunoassay for IL-1α or IL-1β revealed that IL-1β was responsible for IL-1 activity of these culture supernatants. Furthermore, the bone resorbing activities of these culture supernatants were completely neutralized by pretreatment of anti-IL-1β, but not anti-IL-1α antibody. By Northern blot analysis, IL-1β mRNA was identified from these myeloma cells. Therefore, it is concluded that myeloma cells produce IL-1β, which acts as bone-resorbing activity in multiple myeloma.
AB - Human myeloma cells were purified from bone marrow aspirates from four patients having advanced myeloma, including one with common acute lymphoblastic leukemia antigen-positive myeloma. All of these myelomas had marked bone lytic lesions. From the culture supernatants of these purified myeloma cells, bone-resorbing activities were significantly revealed by 45Ca-release bone resorption assay, and IL-1 activities were also detected by IL-1 bioassay (mouse thymocyte comitogenic assay). Sandwich enzyme immunoassay for IL-1α or IL-1β revealed that IL-1β was responsible for IL-1 activity of these culture supernatants. Furthermore, the bone resorbing activities of these culture supernatants were completely neutralized by pretreatment of anti-IL-1β, but not anti-IL-1α antibody. By Northern blot analysis, IL-1β mRNA was identified from these myeloma cells. Therefore, it is concluded that myeloma cells produce IL-1β, which acts as bone-resorbing activity in multiple myeloma.
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U2 - 10.1182/blood.v73.6.1646.1646
DO - 10.1182/blood.v73.6.1646.1646
M3 - Article
C2 - 2785413
AN - SCOPUS:0024599280
VL - 73
SP - 1646
EP - 1649
JO - Blood
JF - Blood
SN - 0006-4971
IS - 6
ER -