Intercalating fluorescence dye YOYO-1 prevents the folding transition in giant duplex DNA

Natsuhiko Yoshinaga, Tatsuo Akitaya, Kenichi Yoshikawa

研究成果: Article査読

24 被引用数 (Scopus)

抄録

Recently, it has become clear that with the addition of polyamines, giant DNA molecules of size greater than 10 kbp exhibit all-or-none switching between elongated coil and folded compact states. Here the effects of the intercalating fluorescent labeling dye, YOYO-1, and the minor-groove binding fluorescent labeling dye, DAPI, on the folding transition of single giant T4 DNA (166 kbp) induced by spermidine3+ were examined, by use of the experimental technique of single molecular chain observation with fluorescence microscopy. It is found that the intercalating dye, YOYO-1, markedly prevents the folding transition, whereas the minor-groove binding dye, DAPI, exhibits negligible effect on the folding transition. This action of YOYO-1 is discussed in relation to the biological effect of intercalators.

本文言語English
ページ(範囲)264-267
ページ数4
ジャーナルBiochemical and biophysical research communications
286
2
DOI
出版ステータスPublished - 2001
外部発表はい

ASJC Scopus subject areas

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学

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