Interaction between Theileria orientalis 23-kDa piroplasm membrane protein and heparin

Hitoshi Takemae, Tatsuki Sugi, Kyousuke Kobayashi, Fumi Murakoshi, Frances C. Recuenco, Akiko Ishiwa, Atsuko Inomata, Taisuke Horimoto, Naoaki Yokoyama, Kentaro Kato

研究成果: Comment/debate査読

2 被引用数 (Scopus)

抄録

The 23-kDa piroplasm membrane protein of Theileria orientalis (p23) is an immunogenic protein expressed during the intraerythrocytic stage of the parasite; its function, however, remains unclear. To evaluate the host factor or factors that interact with p23, we examined the binding of p23 to components of the host cell surface. Recombinant p23 protein of the Ikeda genotype failed to bind to bovine red blood cells or to peripheral blood mononuclear cells, but did bind to Madin-Darby Bovine Kidney (MDBK) cells. A glycoarray assay showed that recombinant p23 proteins from the three genotypes bound to heparin, indicating that p23 is a heparin-binding Theileria surface molecule. Further analysis of heparin-binding molecules is useful for understanding attachment and invasion of T. orientalis merozoites.

本文言語English
ページ(範囲)17-24
ページ数8
ジャーナルJapanese Journal of Veterinary Research
62
1-2
出版ステータスPublished - 2014 1月 1
外部発表はい

ASJC Scopus subject areas

  • 獣医学(全般)

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