Interaction between cells and poly(ethylene glycol)-lipid conjugates

Toru Itagaki, Yusuke Arima, Rei Kuwabara, Narufumi Kitamura, Hiroo Iwata

研究成果: Article査読

12 被引用数 (Scopus)

抄録

Eight types of poly(ethylene glycol)-lipid(PEG-lipids) carrying different lipid tails were synthesized. These PEG-lipids were labeled with fluorescein isothiocyanate (FITC-PEG-lipids) to examine their interaction with cells and to quantitatively determine amounts of PEG-lipids bound on the cell surface. FITC-PEG-lipids spontaneously anchored to the cell membrane within 15min without loss of cell viability. The type of lipid had very little effect on the anchoring rates, while an increase in the hydrophobicity of the lipid portion of the PEG-lipids slowed their dissociation rates. Densities of FITC-PEG-lipids on the cell surface ranged from 1×10-3 to 1×10-2molecules/nm2, depending on the kinds of lipids employed. The relationship between the stability of the lipids on the cell membrane and the hydrophobicity of the lipid moieties will give a basis for the selection of a hydrophobic moiety in PEG-lipid conjugates for use in specific applications.

本文言語English
ページ(範囲)765-773
ページ数9
ジャーナルColloids and Surfaces B: Biointerfaces
135
DOI
出版ステータスPublished - 2015 11 1

ASJC Scopus subject areas

  • バイオテクノロジー
  • 表面および界面
  • 物理化学および理論化学
  • コロイド化学および表面化学

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