Integrative Transformation of Aspergillus oryzae with a Plasmid Containing the Aspergillus nidulans argB Gene

Katsuya Gomi, Yuzuru Iimura, Shodo Hara

研究成果: Article査読

294 被引用数 (Scopus)

抄録

The transformation of Aspergillus oryzae has been achieved with a plasmid carrying the Aspergillus nidulans argB gene coding for ornithine carbamoyltransferase (OCTase). The frequency of transformation was relatively low (0.7 transformants/μg DNA) but the transformed phenotype was extremely stable for many generations without selective pressure. Southern blot analysis revealed that transformation had occurred by integration of multiple tandem copies of plasmid DNA into the host genome through non-homologous recombination. There was no evidence of the existence of free plasmid in the transformants. The number of integrated copies of the plasmid ranged from 15 to 60. The specific activity of OCTase in the cell-free extract was proportional to the copy number of the plasmid, indicating that most of the integrated argB gene was expressed.

本文言語English
ページ(範囲)2549-2555
ページ数7
ジャーナルAgricultural and Biological Chemistry
51
9
DOI
出版ステータスPublished - 1987 1 1
外部発表はい

ASJC Scopus subject areas

  • 生化学、遺伝学、分子生物学(全般)
  • 農業および生物科学(全般)

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