Injection of corticotropin-releasing hormone into the amygdala aggravates visceral nociception and induces noradrenaline release in rats

J. Su, Y. Tanaka, T. Muratsubaki, M. Kano, M. Kanazawa, S. Fukudo

研究成果: Article査読

34 被引用数 (Scopus)

抄録

Background: Corticotropin-releasing hormone (CRH) and its receptor 1 (CRH-R1) play an important role in the colonic response to stress. The central nucleus of the amygdala (CeA) is a major extrahypothalamic site that contains a large number of neurons expressing both CRH and CRH-R1. Here, we verified the hypothesis that CRH in the CeA sensitizes visceral nociception via CRH-R1 with release of noradrenaline, dopamine, and serotonin (5-HT) in the CeA. Methods: In male Wistar rats, visceral sensitivity was quantified by recording the visceromotor response to colorectal distension (CRD) with administration of vehicle, CRH, or the CRH-R1 antagonist CP-154526+ CRH or CRH-R1 antagonist CP-154526 alone into the CeA. Simultaneously, extracellular levels of noradrenaline, dopamine, and 5-HT were measured in the CeA using microdialysis. All data were obtained under restraint conditions. Key Results: Administration of CRH into the CeA significantly increased the number of abdominal muscle contractions in response to CRD. CP-154526 significantly blocked the number of abdominal muscle contractions in response to CRD with the administration of CRH into the CeA. Noradrenaline in the CeA was increased by CRD, further increased by CRH, and inhibited by CRH-R1 antagonist. Dopamine in the CeA was also exaggerated by CRH but was not inhibited by CRH-R1 antagonist. 5-HT in the CeA was unchanged. Conclusions & Inferences: These results suggest that CRH in the CeA sensitizes visceral nociception via CRH-R1 with release of noradrenaline.

本文言語English
ページ(範囲)30-39
ページ数10
ジャーナルNeurogastroenterology and Motility
27
1
DOI
出版ステータスPublished - 2015 1 1

ASJC Scopus subject areas

  • 生理学
  • 内分泌系および自律システム
  • 消化器病学

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