Inhibitory effect of Pseudomonas putida nitrogen-related phosphotransferase system on conjugative transfer of IncP-9 plasmid from Escherichia coli

Kei Inoue, Ryo Miyazaki, Yoshiyuki Ohtsubo, Yuji Nagata, Masataka Tsuda

    研究成果: Letter査読

    6 被引用数 (Scopus)

    抄録

    Conjugative plasmid transfer systems have been well studied, but very little is known about the recipient factors that control horizontal transmission. A self-transmissible IncP-9 naphthalene catabolic plasmid, NAH7, carries the traF gene, whose product is considered to be a host-range modifier of NAH7, because its traF deletion mutant (NAH7dF) is transmissible from Pseudomonas putida to P. putida and Escherichia coli and from E. coli to E. coli, but not from E. coli to P. putida. In this study, transposon mutagenesis of P. putida KT2440 was performed to isolate the mutants that could receive NAH7dF from E. coli. The mutants had the transposon insertions in ptsP or ptsO, encoding two of three components of the nitrogen-related phosphotransferase system (PTSNtr). The KT2440 derivative lacking ptsN, encoding the remaining component of PTSNtr, was also able to receive NAH7dF. These results indicated that the PTSNtr in P. putida is involved in inhibition of conjugative transfer of NAH7dF from E. coli. Our further experiments using site-directed mutants suggested the indirect involvement of the phosphorylated form of PtsO in the inhibition of the conjugative transfer. Conjugative transfer of NAH7 and another IncP-9 plasmid, pWW0, from E. coli was partially inhibited by the PtsO function in KT2440.

    本文言語English
    ページ(範囲)102-109
    ページ数8
    ジャーナルFEMS Microbiology Letters
    345
    2
    DOI
    出版ステータスPublished - 2013 8

    ASJC Scopus subject areas

    • 微生物学
    • 分子生物学
    • 遺伝学

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