Inhibition of light- or glutamate-induced mPer1 expression represses the phase shifts into the mouse circadian locomotor and suprachiasmatic firing rhythms

Masashi Akiyama, Yasuko Kouzu, Satomi Takahashi, Hisanori Wakamatsu, Takahiro Moriya, Miyuki Maetani, Shigenori Watanabe, Hajime Tei, Yoshiyuki Sakaki, Shigenobu Shibata

研究成果: Article査読

239 被引用数 (Scopus)

抄録

mPer1, a mouse gene, is a homolog of the Drosophila clock gene period and has been shown to be closely associated with the light-induced resetting of a mammalian circadian clock. To investigate whether the rapid induction of mPer1 after light exposure is necessary for light-induced phase shifting, we injected an antisense phosphotioate oligonucleotide (ODN) to mPer1 mRNA into the cerebral ventricle. Light-induced phase delay of locomotor activity at CT16 was significantly inhibited when the mice were pretreated with mPer1 antisense ODN 1 hr before light exposure. mPer1 sense ODN or random ODN treatment had little effect on phase delay induced by light pulses. In addition, glutamate-induced phase delay of supra-chiasmatic nucleus (SCN) firing rhythm was attenuated by pretreatment with mPer1 antisense ODN, but not by random ODN. The present results demonstrate that induction of mPer1 mRNA is required for light- or glutamate-induced phase shifting, suggesting that the acute induction of mper1 mRNA in the SCN after light exposure is involved in light-induced phase shifting of the overt rhythm.

本文言語English
ページ(範囲)1115-1121
ページ数7
ジャーナルJournal of Neuroscience
19
3
DOI
出版ステータスPublished - 1999 2 1

ASJC Scopus subject areas

  • Neuroscience(all)

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