Inhibition of H3K18 deacetylation of Sirt7 by Myb-binding protein 1a (Mybbp1a)

Md Fazlul Karim, Tatsuya Yoshizawa, Yoshifumi Sato, Tomohiro Sawa, Kazuhito Tomizawa, Takaaki Akaike, Kazuya Yamagata

研究成果: Article査読

20 被引用数 (Scopus)

抄録

Sirt7 localizes in the nucleus (enriched in the nucleolus) and is an NAD+-dependent deacetylase with high selectivity for the acetylated lysine 18 of histone H3 (H3K18Ac). It has been reported that Sirt7 is necessary for maintaining the fundamental properties of the cancer cell phenotype and stabilizing the tumorigenicity of human cancer via deacetylation of H3K18Ac. However, the regulators of Sirt7 deacetylase activity are unknown. Myb-binding protein 1a (Mybbp1a) is reported to interact with and regulate the function of a number of transcription factors. In the present study, we demonstrated that Mybbp1a binds to Sirt7 in vitro and in vivo. Serial deletion studies indicated that N- and C-terminal regions of Sirt7 and C-terminal region of Mybbp1a are important for the binding. Furthermore, transfection experiments showed that Mybbp1a is capable of inhibiting the deacetylation activity of H3K18Ac by Sirt7. Our findings demonstrate that Mybbp1a is a novel negative regulator of Sirt7.

本文言語English
ページ(範囲)157-163
ページ数7
ジャーナルBiochemical and biophysical research communications
441
1
DOI
出版ステータスPublished - 2013 11 8
外部発表はい

ASJC Scopus subject areas

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学

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