Inhibition of growth hormone secretagogue receptor antagonist, [D-Lys-3]-GHRP-6, in adipogenesis of ovine and rat adipocytes

Sang Gun Roh, Yeon Hee Hong, Daisuke Hishikawa, Hiroaki Tsuzuki, Hisae Miyahara, Yukihiko Nishimura, Chizu Goto, Yasuki Suzuki, Ki Choon Choi, Hong Gu Lee, Shinichi Sasaki

研究成果: Article査読

3 被引用数 (Scopus)

抄録

Ghrelin and growth hormone secretagogues receptor (GHS-R or ghrelin receptor) have been reported as being one of the factors of adipogenesis in adipocytes. To investigate the involvement of ghrelin and GHS-R in adipocytes, the effect of the GHS-R antagonist, [D-Lys-3]-GHRP-6 (His-D-Trp-D-Lys-Trp-D-Phe- Lys-NH 2), on the process of adipogenesis in ovine and rat adipocytes was evaluated. [D-Lys-3]-GHRP-6 (10 -7 mol/L) significantly inhibited adipogenic differentiation of ovine and rat preadipocytes prepared from adipose tissues. The level of peroxisome proliferator activated receptor (PPAR)-γ2 mRNA, an adipogenic marker, was decreased during the differentiation of adipocytes treated with [D-Lys-3]-GHRP-6 for 10 days. Ghrelin stimulated adipogenesis, also causing an increment of glycerol-3-phosphate dehydrogenase and upregulation of PPAR-γ2. Furthermore, the antilipolytic effect of ghrelin was attenuated by treatment with [D-Lys-3]-GHRP-6 in both types of isolated adipocytes. Overall, the results of the present study highlight that GHS-R in adipogenesis can be blocked by treatment with [D-Lys-3]-GHRP-6.

本文言語English
ページ(範囲)381-386
ページ数6
ジャーナルAnimal Science Journal
76
4
DOI
出版ステータスPublished - 2005 8
外部発表はい

ASJC Scopus subject areas

  • 農業および生物科学(全般)

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