Influence of Molecular Sizes of Cryptococcus neoformans Capsular Polysaccharide on Phagocytosis

Akira Yasuoka, Hiroshi Yamada, Mitsuo Kaku, Hironobu Koga, Shigeru Kohno

研究成果: Article査読

28 被引用数 (Scopus)

抄録

The role of capsular polysaccharides (CPS) of Cryptococcus neoformans in phagocytosis by murine alveolar macrophages was investigated in four strains of C. neoformans serotype A, YC-11, YC-5, YC-27 and YC-13. Phagocytosis rates increased markedly after adding 10% mouse serum, compared to fetal calf serum. The reverse relation between capsular thickness of C. neoformans and phagocytosis by alveolar macrophages was observed except in YC-27, which had thin capsules and high virulence. The phagocytosis rate in mice serum was 17.3% in YC-11 (capsule thickness 2.8–3.5 μm), 39.8% in YC-5 (capsule size 0.81.5 μm), 20.3% in YC-27 (capsule size 0.6–1.1 μm), and 62.8% in YC-13 (capsule not detected microscopically). The CPS of YC-11, YC-5, and YC-27 analyzed by gel-filtration using CL-2B showed high molecular fractions near the void volume. However, the CPS of YC-13 showed only low molecular fractions. The widely eluted CPS of YC-11 was separated into 3 fractions and each fraction was added in the phagocytosis assay of YC-13. Phagocytosis was markedly suppressed particularly by the addition of a higher molecular fraction. These results suggest that phagocytosis of C. neoformans by alveolar macrophages is influenced by the molecular sizes of the CPS.

本文言語English
ページ(範囲)851-856
ページ数6
ジャーナルMICROBIOLOGY and IMMUNOLOGY
38
11
DOI
出版ステータスPublished - 1994

ASJC Scopus subject areas

  • 微生物学
  • 免疫学
  • ウイルス学

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