Inflammatory cytokines cause coronary arteriosclerosis-like changes and alterations in the smooth-muscle phenotypes in pigs

Yoshihiro Fukumoto, Hiroaki Shimokawa, Akira Ito, Toshiaki Kadokami, Yoshikazu Yonemitsu, Masanori Aikawa, M. Koji Owada, Kensuke Egashira, Katsuo Sueishi, Ryozo Nagai, Yoshio Yazaki, Akira Takeshita

研究成果: Article査読

66 被引用数 (Scopus)

抄録

We recently developed a porcine model in which chronic, local treatment with interleukin-1β (IL-1β) causes coronary arteriosclerosis-like changes and hyperconstrictive responses. This study was designed to examine whether or not other major inflammatory cytokines [tumor necrosis factor-α (TNF- α) and interleukin-1α (IL-1α)] might also cause similar coronary responses and whether those responses are associated with alterations in the smooth-muscle phenotypes. A segment of the porcine coronary artery was aseptically wrapped with cotton mesh, absorbing IL-1β, TNF-α, and IL-1α. Two weeks after the operation, coronary arteriography showed the development of mild stenotic lesions at the cytokine-treated sites, where hyperconstrictive responses were repeatedly induced by intracoronary serotonin or histamine. Histologically mild intimal thickening was noted at those cytokine-treated sites. Immunostaining and immunoblotting demonstrated that all three myosin heavy chain isoforms, SM1, SM2 (smooth-muscle type), and SMemb (nonmuscle type), were noted in the normal coronary segments, whereas in the segments treated with inflammatory cytokines, SM1 and SM2 were markedly reduced, and only SMemb was noted. These results indicate that inflammatory cytokines all have a similar ability to induce coronary arteriosclerosis-like changes and hyperconstrictive responses, which are associated with alterations in smooth-muscle phenotypes toward dedifferentiation.

本文言語English
ページ(範囲)222-231
ページ数10
ジャーナルJournal of cardiovascular pharmacology
29
2
DOI
出版ステータスPublished - 1997 2

ASJC Scopus subject areas

  • 薬理学
  • 循環器および心血管医学

フィンガープリント

「Inflammatory cytokines cause coronary arteriosclerosis-like changes and alterations in the smooth-muscle phenotypes in pigs」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル