Induction of genomic instability and activation of autophagy in artificial human aneuploid cells

Kentaro Ariyoshi, Tomisato Miura, Kosuke Kasai, Yohei Fujishima, Mitsuo Oshimura, Mitsuaki A. Yoshida

研究成果: Article査読

17 被引用数 (Scopus)

抄録

Chromosome missegregation can lead to a change in chromosome number known as aneuploidy. Although aneuploidy is a known hallmark of cancer cells, the various mechanisms by which altered gene and/or DNA copy number facilitate tumorigenesis remain unclear. To understand the effect of aneuploidy occurring in non-tumorigenic human breast epithelial cells, we generated clones harboring artificial aneuploidy using microcell-mediated chromosome transfer. Our results demonstrate that clones with artificial aneuploidy of chromosome 8 or chromosome 22 both show inhibited proliferation and genomic instability. Also, the increased autophagy was observed in the artificially aneuploidy clones, and inhibition of autophagy resulted in increased genomic instability and DNA damage. In addition, the intracellular levels of reactive oxygen species were up-regulated in the artificially aneuploid clones, and inhibition of autophagy further increased the production of reactive oxygen species. Together, these results suggest that even a single extraneous chromosome can induce genomic instability, and that autophagy triggered by aneuploidy-induced stress is a mechanism to protect cells bearing abnormal chromosome number.

本文言語English
ページ(範囲)19-30
ページ数12
ジャーナルMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis
790
DOI
出版ステータスPublished - 2016 8月 1
外部発表はい

ASJC Scopus subject areas

  • 分子生物学
  • 遺伝学
  • 健康、毒物学および変異誘発

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