Induction of cross‐fertilization between sea urchin eggs and starfish sperm by polyethylene glycol treatment

Keiichiro Kyozuka, Kenzi Osanai

研究成果: Article査読

3 被引用数 (Scopus)

抄録

Cross‐fertilization between sea urchin eggs (Strongylocentrotus nudus) and starfish sperm (Asterina pectinifera) was induced by treatment with polyethylene glycol (PEG). Without treatment with PEG, the denuded egg surface (jelly coat‐ and vitelline coat‐free) engulfed the head of acrosome‐reacted sperm; however, sperm penetration did not occur [Kyozuka and Osanai, 1988]. When these eggs were exposed briefly to PEG (molecular weight 3,000) in seawater, the sperm entered the egg by membrane fusion. Cortical granules were discharged, and embryogenesis began following sperm penetration. PEG did not induce parthenogenesis in Strongylocentrotus eggs. Egg activation is thus closely linked with gamete membrane fusion.

本文言語English
ページ(範囲)123-129
ページ数7
ジャーナルGamete Research
22
2
DOI
出版ステータスPublished - 1989 2

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology

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